Li Y, Ku CY, Xu J, Saxena D, Caufield PW (2005) Survey of oral microbial diversity using PCR-based denaturing gradient gel electrophoresis. Siqueira JF Jr, Rôças IN, Rosado AS (2005) Application of denaturing gradient gel electrophoresis (DGGE) to the analysis of endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 98:741–749 Rôças IN, Siqueira JF Jr, Aboim MC, Rosado AS (2004) Denaturing gradient gel electrophoresis analysis of bacterial communities associated with failed endodontic treatment. Siqueira JF Jr, Rôças IN, Rosado AS (2004) Investigation of bacterial communities associated with asymptomatic and symptomatic endodontic infections by denaturing gradient gel electrophoresis fingerprinting approach. Sakamoto M, Huang Y, Ohnishi M, Umeda M, Ishikawa I, Benno Y (2004) Changes in oral microbial profiles after periodontal treatment as determined by molecular analysis of 16S rRNA genes. Zijnge V, Harmsen HJ, Kleinfelder JW, van der Rest ME, Degener JE, Welling GW (2003) Denaturing gradient gel electrophoresis analysis to study bacterial community structure in pockets of periodontitis patients. Sakamoto M, Takeuchi Y, Umeda M, Ishikawa I, Benno Y (2003) Application of terminal RFLP analysis to characterize oral bacterial flora in saliva of healthy subjects and patients with periodontitis. Denaturing gradient gel electrophoresis (DGGE).Terminal restriction fragment length polymorphism (T-RFLP).This chapter focuses on two particular techniques, namely, terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE), both of which have been widely used in environmental studies and have been successfully used by the authors in the study of the oral microbial communities associated with conditions of health and disease. The application of molecular biology approaches holds the advantage of including culture-difficult and as-yet-uncultivated phylotypes in the profiles, providing a more comprehensive picture of the microbial community. Profiling analysis of microbial communities has recently assumed special relevance as it allows a thorough understanding of the diversity of the microbiota, its behavior over time, and the establishment of patterns associated with health and disease. In their natural environments, microorganisms usually live in organized communities.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |